Principal Name: WNT4

$295.00

Code: CE0598

Synonyms: WNT4 ; LEF1; lymphoid enhancer-binding factor 1 ; HGNC:6551; DKFZp586H0919; TCF1ALPHA ; lymphoid enhancer binding factor-1

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CE0598: Staining (0.03µg/ml) of a) Human Ovary and b) Human Testes (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Specifications print
Amount:	100 ug
Host:	Goat
Antigen:	Peptide with sequence C-SNWLYLAKLSSVGS, from the internal region of the protein sequence according to NP_110388.2
Purification:	Affinity Purification
Species Reactivity:	Human
Buffer & Formulation:	Supplied as 100µg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Storage:	Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone:	polyclonal

Applications
ELISA:	1:32,000
Western Blotting:	0.03-0.1µg/ml
IHC:
Immunoprecipitation:
Flow Cytometry:

Comments:

Western Blot: Approx 35kDa band observed in lysates of Human Testes and Ovary (calculated MW of 39.1kDa according to NP_110388.2). Recommended concentration: 0.03-0.1µg/ml

Expected reactivity from sequence similarity: Human, Mouse, Rat

References:

Huguet EL, McMahon JA, McMahon AP, Bicknell R, Harris AL. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell. Cancer Res. 1994 May 15;54(10):2615-21. PMID: 8168088 Abstract

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