Principal Name: Amphiphysin / AMPH

$295.00

Code: CE0599

Synonyms: Amphiphysin / AMPH ; AMPH; amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen) ; HGNC:471; AMPH1; amphiphysin; amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen)

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CE0599: (0.5µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications print
Amount:	100 ug
Host:	Goat
Antigen:	Peptide with sequence C-KRKDESRISKAE, from the internal region of the protein sequence according to NP_001626.1; NP_647477.1
Purification:	Affinity Purification
Species Reactivity:	Mouse
Buffer & Formulation:	Supplied as 100µg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Storage:	Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone:	polyclonal

Applications
ELISA:	1:4,000
Western Blotting:	0.1-0.3µg/ml
IHC:
Immunoprecipitation:
Flow Cytometry:

Comments:

This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).

Western Blot: Approx 125kDa band observed in mouse brain lysates (calculated MW of 76.3kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997 Oct;8(10):2003-15.; PMID: 9348539). Recommended concentration: 0.1-0.5µg/ml.
A minor band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide.

Expected reactivity from sequence similarity: Human, Mouse, Rat, Dog

References:

Sommer C, Weishaupt A, Brinkhoff J, Biko L, Wessig C, Gold R, Toyka KV. Paraneoplastic stiff-person syndrome: passive transfer to rats by means of IgG antibodies to amphiphysin. Lancet. 2005 Apr;365(9468):1406-11. PMID: 15836889 Abstract

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