Principal Name: CD14

$295.00

Code: CE0610

Synonyms: CD14 ; UBE2V1; ubiquitin-conjugating enzyme E2 variant 1; CIR1; UEV1; CROC1; UEV-1; UEV1A; CROC-1; DNA-binding protein

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CE0610: (1µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications print
Amount:	100 ug
Host:	Goat
Antigen:	Peptide with sequence PGEVQASYLKSQS-C, from the N Terminus of the protein sequence according to NP_068823.2; NP_954595.1.
Purification:	Peptide Affinity Purification
Species Reactivity:	Human
Buffer & Formulation:	Supplied as 100µg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Storage:	Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone:	polyclonal

Applications
ELISA:
Western Blotting:	0.2-1µg/ml
IHC:
Immunoprecipitation:
Flow Cytometry:

Comments:

Western Blot: Approx 55kDa band observed in Human Lung, Lymph Node and Tonsil lysates (calculated MW of 40.1kDa according to NP_000582.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun;69(6):3772-81; PMID: 11349042). Recommended concentration: 1-3µg/ml.

References:

Rothofsky ML, Lin SL.
CROC-1 encodes a protein which mediates transcriptional activation of the human FOS promoter.
Gene. 1997 Aug 22;195(2):141-9. Abstract
PMID: 9305758

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