Principal Name: NGFR

$295.00

Code: CE0611

Synonyms: NGFR ; NGFR; nerve growth factor receptor (TNFR superfamily, member 16) ; HGNC:7809; TNFRSF16; p75(NTR); p75NTR

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CE0611: (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications print
Amount:	100 ul
Host:	Goat
Antigen:	Peptide with sequence C-AKREEVEKLLNG, from the internal region of the protein sequence according to NP_002498.1.
Purification:	Affinity Purification
Species Reactivity:	Human
Buffer & Formulation:	Supplied as 100µg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Storage:	Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone:	polyclonal

Applications
ELISA:
Western Blotting:	1-3µg/ml
IHC:
Immunoprecipitation:
Flow Cytometry:

Comments:

Western Blot: Approx 75kDa band observed in Human Duodenum and Human Heart lysates (calculated MW of 45.2kDa according to NP_4804). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nykjaer et al, Curr Opin Neurobiol. 2005 Feb;15(1):49-57). Recommended concentration: 0.3-1µg/ml.

Expected reactivity from sequence similarity: Human, Mouse, Rat, Dog

References:

Nakamura T, Endo K, Kinoshita S. Identification of human oral keratinocyte stem/progenitor cells by neurotrophin receptor p75 and the role of neurotrophin/p75 signaling. Stem Cells. 2007 Mar;25(3):628-38. Epub 2006 Nov 16. PMID: 17110619 Abstract

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