Principal Name: Survivin / BIRC5

$295.00

Code: CE0612

Synonyms: Survivin / BIRC5 ; PYCARD; PYD and CARD domain containing; ASC; CARD5; MGC10332; TMS1; apoptosis-associated speck-like protein containing a CARD; caspase recruitment domain protein 5; target of methylation-induced silencing-1

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CE0612: (0.01µg/ml) staining of Molt-4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications print
Amount:	100 ul
Host:	Goat
Antigen:	Peptide with sequence CVRRAIEQLAAMD, from the C Terminus of the protein sequence according to NP_001159.2; NP_001012271.1.
Purification:	Affinity Purification
Species Reactivity:	Human
Buffer & Formulation:	Supplied as 100µg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Storage:	Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone:	polyclonal

Applications
ELISA:	1:32,000
Western Blotting:	0.2-1µg/ml
IHC:
Immunoprecipitation:
Flow Cytometry:

Comments:

This antibody is expected to recognize the reported isoforms 1 and 3 (NP_001159.2 and NP_001012271.1 resp.)

Western Blot: Approx 18kDa band observed in Human T cell line MOLT4 lysates (calculated MW of 16.4kDa according to NP_001159.2 and 18.6kDa according to NP_001012271.1). Recommended concentration: 0.01-0.5µg/ml.

References:

Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC, Altieri DC. Control of apoptosis and mitotic spindle checkpoint by survivin. Nature. 1998 Dec 10;396(6711):580-4. PMID: 9859993 Abstract

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