Principal Name: Bin3

$295.00

Code: CE0816

Synonyms: MEPCE; methylphosphate capping enzyme; BCDIN3; FLJ20257; bin3, bicoid-interacting 3; bin3, bicoid-interacting 3, homolog

larger image

CE0816: 0.3µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications print
Amount:	100 ug
Host:	Goat
Antigen:	Peptide with sequence C-TPHNTSKGFQRP, from the internal region of the protein sequence according to NP_062552.2.
Purification:	Peptide Affinity Purification
Species Reactivity:	Human
Buffer & Formulation:	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage:	Aliquot and store at -20°C. Minimize freezing and thawing.
Clone:

Applications
ELISA:	1:16000
Western Blotting:	0.3-1µg/ml
IHC:
Immunoprecipitation:
Flow Cytometry:

Comments:

Western Blot: Approx. 75kDa band observed in lysates of cell line K562 (calculated MW of 74.4kDa according to NP_062552.2). Recommended concentration: 0.3-1µg/ml.

References:

Jeronimo C, Forget D, Bouchard A, Li Q, Chua G, Poitras C, Thérien C, Bergeron D, Bourassa S, Greenblatt J, Chabot B, Poirier GG, Hughes TR, Blanchette M, Price DH, Coulombe B.
Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme.
Mol Cell. 2007 Jul 20;27(2):262-74.
PMID: 17643375 Abstract

id#: 878