Principal Name: Annexin A11

$295.00

Code: CE0546

Synonyms: Annexin A11 ; GRB2; growth factor receptor-bound protein 2; ASH; ASH protein; EGFRBP-GRB2; Grb3-3; MST084; MSTP084; HT027; abundant SRC homology; epidermal growth factor receptor-binding protein GRB2; growth factor receptor-bound protein 3

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CE0546: Staining (0.2ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Specifications print
Amount:	100 ug
Host:	Goat
Antigen:	Peptide with sequence YPGYPPPPGGYPP-C, from the N Terminus of the protein sequence according to NP_001148.1; NP_665875.1; NP_665876.1
Purification:	Affinity Purification
Species Reactivity:	Human
Buffer & Formulation:	Supplied as 100µg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Storage:	Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone:	polyclonal

Applications
ELISA:	1:32,000
Western Blotting:	0.2-1µg/ml
IHC:
Immunoprecipitation:
Flow Cytometry:

Comments:

NP_001148.1; NP_665875.1 and NP_665876.1 are varients that encode the same protein.

Western Blot: Approx 55kDa band seen in HeLa lysate (Predicted MW of approx. 58kDa according to NP_001148.1). Recommended for use at 0.2-1µg/ml.

References:

Misaki Y, Pruijn GJ, van der Kemp AW, van Venrooij WJ. The 56K autoantigen is identical to human annexin XI. J Biol Chem. 1994 Feb 11;269(6):4240-6. PMID: 7508441 Abstract

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