Principal Name: Calretinin

$295.00

Code: CE0547

Synonyms: Annexin A2 ; VAV2; vav 2 oncogene; Oncogene VAV2

larger image

CE0547: Staining (1µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Specifications print
Amount:	100 ug
Host:	Goat
Antigen:	Peptide with sequence STVHEILCKLSLEGD, from the N Terminus of the protein sequence according to NP_001002858.1; NP_004030.1; NP_001002857.1
Purification:	Affinity Purification
Species Reactivity:	Human
Buffer & Formulation:	Supplied as 100µg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Storage:	Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone:	polyclonal

Applications
ELISA:	1:4,000
Western Blotting:	1-3µg/ml
IHC:
Immunoprecipitation:
Flow Cytometry:

Comments:

This antibody is expected to recognise both reported isoforms. NP_004030.1 and NP_001002857.1 are varients of isoform 2.

Western Blot: Approx 35kDa band observed in lysates of cell lines A431 and A549 (calculated MW of 38.6kDa according to NP_004030.1 and NP_001002857.1). Recommended concentration: 1-3µg/ml.

Expected reactivity from sequence similarity: Human, Mouse, Rat, Cow, Pig

References:

Takahashi S, Reddy SV, Chirgwin JM, Devlin R, Haipek C, Anderson J, Roodman GD. Cloning and identification of annexin II as an autocrine/paracrine factor that increases osteoclast formation and bone resorption. J Biol Chem 1994 Nov 18;269(46):28696-701 PMID: 7961821 Abstract

id#: 446