Principal Name: ARH / LDL receptor adaptor

$295.00

Code: CE0548

Synonyms: ARH / LDL receptor adaptor ; RAB11A; YL8; RAB11A, member RAS oncogene family; RAB 11A, member oncogene family

larger image

CE0548: Staining (3µg/ml) of Human Liver lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Specifications print
Amount:	100 ug
Host:	Goat
Antigen:	Peptide with sequence DALKSAGRALIRS-C, from the N Terminus of the protein sequence according to NP_056442.1Peptide with sequence DALKSAGRALIRS-C, from the N Terminus of the protein sequence according to NP_056442.1
Purification:	Affinity Purification
Species Reactivity:	Human
Buffer & Formulation:	Supplied as 100µg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Storage:	Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone:	polyclonal

Applications
ELISA:	1:16,000
Western Blotting:	1-3µg/ml
IHC:
Immunoprecipitation:
Flow Cytometry:

Comments:

Western Blot: Approx 40-45kDa band observed in Human Liver lysate (predicted MW of 35kDa according to NP_056442.1). Recommended for use at 1-3µg/ml.

Expected reactivity from sequence similarity: Human, Mouse

References:

He G, Gupta S, Yi M, Michaely P, Hobbs HH, Cohen JC. ARH is a modular adaptor protein that interacts with the LDL receptor, clathrin, and AP-2. J Biol Chem. 2002 Nov 15;277(46):44044-9. PMID: 12221107 Abstract

id#: 447