Principal Name: BLNK

$295.00

Code: CE0549

Synonyms: BLNK ; RPS6KB1; ribosomal protein S6 kinase, 70kD, polypeptide 1; PS6K; S6K; S6K1; STK14A; p70(S6K)-alpha; p70-S6K; p70-alpha; p70 S6 kinase, alpha 1; p70 S6 kinase, alpha 2; ribosomal protein S6 kinase, 70kD, polypeptide 1; serine/threonine kinase 14 alpha

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CE0549: Staining (4µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Specifications print
Amount:	100 ug
Host:	Goat
Antigen:	Peptide with sequence C-KDSTRLKYAVKVS, from the C Terminus of the protein sequence according to NP_037446.1
Purification:	Affinity Purification
Species Reactivity:	Human
Buffer & Formulation:	Supplied as 100µg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Storage:	Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone:	polyclonal

Applications
ELISA:
Western Blotting:	3-5µg/ml
IHC:
Immunoprecipitation:
Flow Cytometry:

Comments:

Western blot: Approx 70kDa band seen in Daudi lysate. Recommended for use at 3-5µg/ml. Please note that although the predicted MW of BLNK is approximately 50kDa, it runs at 70-75kDa on SDS-PAGE.

Expected reactivity from sequence similarity: Human, Mouse, Rat

References:

Fu C, Turck CW, Kurosaki T, Chan AC. BLNK: a central linker protein in B cell activation. Immunity. 1998 Jul;9(1):93-103. PMID: 9697839 Abstract

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