Principal Name: CSK

$295.00

Code: CE0551

Synonyms: CSK ; RXRB; NR2B2; DAUDI6; RCoR-1; MGC1831; H-2RIIBP; retinoid X receptor, beta; MHC class I promoter binding protein

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CE0551: Staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Specifications print
Amount:	100 ug
Host:	Goat
Antigen:	Peptide with sequence C-EQLEHIKTHELHL, from the C Terminus of the protein sequence according to NP_004374.1
Purification:	Affinity Purification
Species Reactivity:	Human
Buffer & Formulation:	Supplied as 100µg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Storage:	Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone:	polyclonal

Applications
ELISA:	1:32,000
Western Blotting:	0.5-2µg/ml
IHC:	10ug/ml
Immunoprecipitation:	0.1-0.3ug/ml
Flow Cytometry:

Comments:

Western Blot: Approx 50kDa band observed in Jurkat lysate (predicted MW of 50.7kDa according to NP_004374.1). Recommended for use at 0.5-2µg/ml.
Immunohistochemistry: In paraffin embedded human tonsil, strong staining of germinal centre cells and less intense labelling in the interfollicular area. Recommended concentration 0.1-0.3ug/ml

Expected reactivity from sequence similarity: Human, Mouse, Rat

References:

Holtrich U, Brauninger A, Strebhardt K, Rubsamen-Waigmann H. Two additional protein-tyrosine kinases expressed in human lung: fourth member of the fibroblast growth factor receptor family and an intracellular protein-tyrosine kinase. Proc Natl Acad Sci U S A 1991 Dec 1;88(23):10411-5 PMID: 1720539 Abstract

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