Principal Name: Ezrin / villin 2

$295.00

Code: CE0552

Synonyms: Ezrin / villin 2 ; AKT3; PKBG; PRKBG; STK-2; RAC-gamma; RAC-PK-gamma; DKFZP434N0250; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); protein kinase B gamma; serine threonine protein kinase, Akt-3; RAC-gamma serine/threonine protein kinase

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CE0552: (1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications print
Amount:	100 ug
Host:	Goat
Antigen:	Peptide with sequence C-QDYEEKTKKAER, from the internal region of the protein sequence according to NP_003370.2
Purification:	Affinity Purification
Species Reactivity:	Human Mouse
Buffer & Formulation:	Supplied as 100µg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Storage:	Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone:	polyclonal

Applications
ELISA:	1:16,000
Western Blotting:	1-3µg/ml
IHC:
Immunoprecipitation:
Flow Cytometry:

Comments:

Western Blot: Approx 80kDa band observed in Human Duodenum and Placenta lysates (calculated MW of 69.4kDa according to NP_003370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhu et al, Am J Physiol Cell Physiol. 2005 Jun;288(6):C1242-54.; PMID: 15788482). Recommended concentration: 1-3µg/ml.

Expected reactivity from sequence similarity: Human, Dog

References:

Stanasila L, Abuin L, Diviani D, Cotecchia S. Ezrin directly interacts with the alpha1b-adrenergic receptor and plays a role in receptor recycling. J Biol Chem. 2006 Feb 17;281(7):4354-63. Epub 2005 Dec 13. PMID: 16352594 Abstract

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