Principal Name: FYN

$295.00

Code: CE0553

Synonyms: FYN ; VDR; vitamin D (1,25- dihydroxyvitamin D3) receptor ; HGNC:12679; NR1I1; vitamin D (1,25-dihydroxyvitamin D3) receptor

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CE0553: Staining 0.2µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Specifications print
Amount:	100 ug
Host:	Goat
Antigen:	Peptide with sequence VQCKDKEATKLTE, from the N Terminus of the protein sequence according to NP_002028.1; NP_694592.1; NP_694593.1
Purification:	Affinity Purification
Species Reactivity:	Human Mouse
Buffer & Formulation:	Supplied as 100µg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Storage:	Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone:	polyclonal

Applications
ELISA:	1:32,000
Western Blotting:	0.2-1µg/ml
IHC:
Immunoprecipitation:
Flow Cytometry:

Comments:

This antibody is expected to recognise all three reported isoforms (as represented by NP_002028.1; NP_694592.1; NP_694593.1).

Western Blot: Approx 55-60kDa band observed in Jurkat, A431 and NIH-3T3 lysates (predicted MW of 62kDa according to NP_002028.1). Recommended for use at 0.2-1µg/ml.

Expected reactivity from sequence similarity: Human, Mouse, Rat

References:

Semba K, Nishizawa M, Miyajima N, Yoshida MC, Sukegawa J, Yamanashi Y, Sasaki M, Yamamoto T, Toyoshima K. yes-related protooncogene, syn, belongs to the protein-tyrosine kinase family. Proc Natl Acad Sci U S A 1986 Aug;83(15):5459-63 PMID: 3526330 Abstract

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