Principal Name: MYD88

$295.00

Code: CE0613

Synonyms: MYD88 ; IRAK4; REN64; NY-REN-64; interleukin-1 receptor-associated kinase 4; IRAK-4 mutated form 1; interleukin-1 receptor associated kinase 4; interleukin-1 receptor associated kinase 4 mutant form 1

larger image

CE0613: Staining (0.01µg/ml) of MOLT4 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Specifications print
Amount:	100 ug
Host:	Goat
Antigen:	Peptide with sequence C-IKYKAMKKEFP, from the internal region of the protein sequence according to NP_002459.1.
Purification:	Peptide Affinity Purification
Species Reactivity:	Human
Buffer & Formulation:	Supplied as 100µg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Storage:	Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone:	polyclonal

Applications
ELISA:	1:64,000
Western Blotting:	0.01-0.03µg/ml
IHC:	2-5µg/ml
Immunoprecipitation:
Flow Cytometry:

Comments:

Western Blot: Approx 35kDa band observed in Human Tcell line MOLT4 lysates (calculated MW of 33.2kDa according to NP_002459). Recommended concentration: 0.01-0.03µg/ml.
Immunohistochemistry: In paraffin embedded tonsil, stains clusters of cells some of which are likely to be plasma cells. In the interfollicular area occasional strongly stained areas. Epithelium is weakly positive. Recommended concentration, 2-5µg/ml.

Expected reactivity from sequence similarity: Human, Rat, Mouse, Dog, Chimpanzee

References:

Li X, Qin J. Modulation of Toll-interleukin 1 receptor mediated signaling. J Mol Med. 2005 Jan 21; Abstract PMID: 15662540

id#: 505